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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become noticed in many tumors, which results in chromosome instability, and therefore may well contribute to tumorigenesis. This protein was located to affiliate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a significant role in advertising and marketing mobile-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, have already been described. Two added splice variants had been documented but in-depth nucleotide sequence information and facts will not be nonetheless out there.
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Blocking peptides are peptides that bind particularly on the concentrate on antibody and block antibody binding. These peptide usually has the epitope recognized because of the antibody.
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This guideline outlines further more possible optimization of mobile and immunoassay parameters to be certain the absolute best final results are...
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This information outlines even further doable optimization of mobile and immunoassay parameters to be certain the very best success are...
Antibodies sure to the blocking peptide now not bind into the epitope over the concentrate on protein. By comparing the staining through the blocked antibody as opposed to the antibody on your own, one can see which staining is certain.
Goal Info Cyclin E belongs towards the highly conserved cyclin family, whose users are characterised by a remarkable periodicity in protein abundance through the mobile cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit distinctive expression and degradation designs which lead to the temporal coordination of each and every mitotic function. Cyclin E types a posh with and features like a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates on the G1-S phase boundary which is degraded as cells progress as a result of S period.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by the two antibodies...
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